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. 2007 Jul 23;2:85–97.

Figure 3.

Figure 3

ILK inhibition suppresses c-Jun activation. A) MDA-MB-231 cells were treated with 25 μM LY294002, 12.5 μM QLT0267 or 25 μM QLT0267. Cells were fractionated to separate cytoplasmic (C) and nuclear (N) compartments and analyzed by western blotting. Phospho-c-Jun (Ser63) expression decreased when treated with QLT0267. B) Treatment with 25 nM ILK siRNA, inhibited phospho-c-Jun (Ser63) expression while there was no effect on total c-Jun. C) Cells were serum starved (lane 1) or stimulated with IGF-1 to induce c-Jun phosphorylation (lane 2). The addition of LY294002 (20 μM) or PD98059 (20 μM, lanes 3&4) partially inhibited c-Jun phosphorylation following IGF-1 stimulation. Both compounds maximally attenuated the phosphorylation of c-Jun (lane 5).