Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Feb 8;6(3):710–720. doi: 10.1242/dmm.011445

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

PMC Copyright notice

Fig. 7. — mTOR inhibition reduces endometrial lesions and thyroid hyperplasia. (A) Schematic representation of the protocol used for everolimus administration. Briefly, 5 weeks after tamoxifen injection, animals started to receive a single daily intraperitoneal dose of everolimus for 14 consecutive days and then they were sacrificed. (B) At 7 weeks after tamoxifen injection (TAM), mice exhibited lethargy, ruffled fur and hunched posture. Animals treated with everolimus (TAM+EVE) remained healthy and active, similar to controls (CON). (C) H&E staining and PTEN immunohistochemistry images corresponding to endometria from mice treated with corn oil (CON), tamoxifen (TAM) or tamoxifen plus everolimus (TAM+EVE). Everolimus-treated females showed reduced lesions and PTEN-negative healthy areas. Percentage of animals developing endometrial hyperplasia (EH) and endometrial in situ adenocarcinomas (EC) after injection of corn oil (CON), tamoxifen (TAM) or tamoxifen and everolimus (TAM+EVE) are shown in the top graph. Representative images (bottom left) and quantification (bottom right) of Ki-67 immunostaining performed on uterus dissected from mice injected with corn oil (CON), tamoxifen (TAM) or tamoxifen plus everolimus (TAM+EVE). (D) Representative images of thyroids dissected from mice injected with corn oil (CON), tamoxifen (TAM) or tamoxifen plus everolimus (TAM+EVE). Everolimus treatment also reduced thyromegaly in tamoxifen-injected mice. Everolimus caused a marked decrease of the index of proliferation, with levels similar to control animals, as assessed by Ki-67 immunostaining. *P≤0.05; **P≤0.001.