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. 2013 Feb 1;6(3):721–733. doi: 10.1242/dmm.010710

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 2. — TDP-43 forms axonal aggregates in developing eyes. (A–H) Confocal images (single slices, 1 μm each or 2–3 slice projections) showing GFP NLS (A,E) or TDP-43 variant (B–D,F–H) localization when expressed in the developing eye neuroepithelium with GMR-Gal4. TDP-43 visualized via individual fluorescent YFP tags (indicated as GFP here). Filamentous actin labeled with phalloidin, DNA stained with Hoechst 33342 as indicated. Note TDP-43 puncta in axons (arrowheads in B–D,F–H and insets); compare with GFP NLS controls (arrowheads in A,E and inset). (I) Cellular fractionation of adult head tissue shows TDP-43 distribution complexes ranging from highly soluble (LS, low salt) to insoluble (U, urea). Triton X-100 (TX) and sarkosyl (SK) correspond to non-ionic and ionic detergent soluble fractions, respectively. TDP-43 was detected using anti-GFP antibodies. Tubulin was used as a loading control. Scale bar: 75 μm.