Fig. 4.

TDP-43 is expressed in dynamic cytoplasmic puncta in primary motor neurons. (A–F) TDP-43 expressed using D42 Gal4, visualized with anti-GFP antibody, localizes to distinct puncta within neurites (arrows). Neuronal membranes were labeled with anti-HRP antibodies. (G) Velocity quantification of TDP-43-containing puncta (mean, maximum and minimum velocities). (H) Quantification of total and net distances for TDP-43 puncta. (I) FRAP indicates that TDP-43 is mobile within neurites. Note that wild-type kinetics are significantly different to those of mutant TDP-43 (see text for discussion). Values show means ± s.e.m. Scale bar: 15 μm.