Fig. 3.
Comparison of the innate immune response of myd88−/− and wild-type embryos to E. tarda and S. typhimurium. Gene expression profiles of myd88−/− and wild-type embryos infected with E. tarda FL6-60 or S. typhimurium SL1027 are depicted in a heat map. Embryos were infected with 150 cfu of either pathogen into the caudal vein at 28 hpf and snap-frozen individually at 8 hpi. Triplicate samples for each infection condition were compared with samples from control embryos (injected with PBS) using a common reference microarray design. Immune-related genes in the heat map are ordered in functional groups. All genes included in the heat map are represented by a minimum of two probes that showed significant up- or downregulation (significance cut-offs for the ratios of infected versus control groups were set at twofold with P<10−5). Up- and downregulation is indicated by increasingly bright shades of yellow and blue, respectively. All genes listed in this figure are named according to sequence homology with mammalian counterparts and in most cases have not yet been confirmed functionally.