Fig. 6.

Bacterial .burdens of myd88^−/− and wild-type embryos infected with M. marinum. (A–F) Representative stereo fluorescence images of infected embryos. At 28 hpf, myd88^−/− (Mu) and wild-type (Wt) embryos were infected with ∼200 cfu of mCherry-labeled M. marinum strain Mma 20 (A,B) or strain E11 (C,D) by injection into the blood island. Stereo fluorescence images were taken at 3 (Mma20 strain) or 5 dpi (E11 strain). For comparison, wild-type and myd88-morpholino-injected embryos were infected with the same dose of M. marinum Mma 20 (E,F). (G–I) Quantification of bacterial burden. Red symbols indicate which infected individuals are shown as representative images in A–F. Bacterial pixel counts were determined based on stereo fluorescence images. Significant differences were determined by one-way ANOVA with Tukey’s Multiple Comparison method as a post-hoc test; ***P<0.001.