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. 2013 Apr 10;6(3):855–865. doi: 10.1242/dmm.011759

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 1. — Evaluation of raw scores of control groups in historical studies. The mean and standard deviations of the manual (A) and automated (B) raw scores for equivalent (Ø, −ve and +ve) control groups in each study. The bars indicate the mean score within the group, whereas the error bars indicate 1 s.d. The mean score across all studies for the Ø control groups divided by the mean score of the animals in each corresponding −ve control group is 0.005±0.016 (manual) and 0.430±0.098 (automated). The corresponding values for the −ve control group are 1±0.186 (manual) and 1±0.178 (automated). Finally, within the +ve control group, the corresponding values are 0.506±0.088 (manual) and 0.584±0.160 (automated). The relatively large s.d. of mean scores from equivalent control groups suggest that the raw scores from different studies need to be normalized in order to be comparable.