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. Author manuscript; available in PMC: 2014 May 25.
Published in final edited form as: Virology. 2013 Mar 14;440(1):89–96. doi: 10.1016/j.virol.2013.02.009

Figure 1. Feeding of Cells with Neu5Gc but not Neu5Ac induces Sialic Acid-dependent Parvovirus Capsid binding.

Figure 1

The canine MDCK, canine A72 and human 293 cells were incubated with 3 mM Neu5Gc or Neu5Ac for three days to allow metabolic incorporation and then exposed to biotinylated CPV (dashed line), FPV (solid line) or no virus (only fluorescent streptavidin; shaded grey) capsids at pH 6.2 at 4°C. Binding of capsids was detected by fluorescent Streptavidin binding, using flow cytometry.