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. 2013 Mar 19;25(3):1016–1028. doi: 10.1105/tpc.113.110106

Figure 6.

Figure 6.

14-3-3 Stabilizes ACS Proteins via Downregulation of an ETO1/EOL E3 Ligase-Independent Pathway.

(A) Triple response phenotype of etiolated eto1/eol1/eol2 seedlings grown in the presence of no peptide or 200 µg/mL R18 or R18Lys peptide. Bar = 1 mm.

(B) Hypocotyl lengths of 3-d-old etiolated eto1/eol1/eol2 seedlings grown in the presence of no peptide or 200 µg/mL R18 or R18Lys peptide. n ≥ 18; mean ± se; *P < 0.001 (Student’s t test).

(C) Ethylene production from etiolated eto1/eol1/eol2 seedlings grown in the presence or absence of R18 or R18Lys peptide. Mean ± se; n = 3; *P < 0.02 (Student’s t test).

(D) and (E) R18 peptide induces destabilization of myc-ACS5 proteins in eto1/eol1/eol2 background. eto1/eol1/eol2 (D) or wild-type (E) protoplasts were cotransfected with a plasmid expressing myc-ACS5 and incubated with the indicated concentrations of R18 or R18Lys peptide. Total proteins were analyzed by immunoblotting using an anti-myc or anti-GFP antibody. Right: The myc-ACS5 signals were normalized to the matching GFP signals, and these values expressed relative to the 0 concentration of R18, which was set to 1.