Figure 1.

The Double Knockout ysl4 ysl6 Has Decreased Tolerance to Elevated Iron Levels.

(A) Position and orientation of the T-DNA insertion site for the ysl4-1 (SALK_025447) and ysl6-1 (SALK_093392) knockout mutants.

(B) Relative YSL4 and YSL6 transcript levels in wild-type, single ysl4-1 and ysl6-1, and double ysl4-1 ysl6-1 knockout plants grown for 15 d in standard 0.5× Murashige and Skoog medium, assessed by quantitative RT-PCR. nd, not detectable; WT, the wild type. Error bars represent the se of two independent experiments.

(C) Phenotype of wild-type, ysl4-1, ysl6-1, and ysl4-1 ysl6-1 plants grown for 15 d in iron-replete (50 µM) or iron-excess (500 µM) conditions.

(D) Growth restoration of ysl4-1 ysl6-1 double mutant plants complemented with a functional copy of YSL6 (Pro35S:YSL6 #1).

[See online article for color version of this figure.]