Figure 6.

Iron Content Is Increased in the Chloroplasts of the ysl4 ysl6 Double Mutant.

(A) to (F) Perls/DAB staining of iron in leaf sections prepared from 3-week-old soil-grown plants irrigated with water ([A] and [B]) or 500 µM FeEDDHA (+Fe; [C] to [F]). (E) and (F) are higher magnification images of (C) and (D), respectively. Position of the veins (v) is indicated by a bracket. Bars = 20 µm in (A) to (D) and 10 µm in (E) and (F).

(A), (C), and (E) The wild type (WT).

(B), (D), and (F) ysl4 ysl6.

(G) ICP-MS measurement of iron content in isolated chloroplasts from leaves of plants grown 3 weeks in soil in short-day conditions. Values represent the mean of three measurements from one experiment representative of two biological replicates. Error bars represent sd.