Figure 7.

Subcellular Localization of As-MT1, As-UGT74H5, and As-SCPL1.

(A) to (D) Transmission electron microscopy images from immunogold labeling of ultrathin sections probed with antisera raised against MT1.

(A) and (B) Root tip epidermal cells of 3-d-old wild-type (WT) (A) and sad9 (#961) mutant (B) oat seedlings.

(C) and (D) Sections from N. benthamiana leaves expressing the CPMV-AsMT1 construct (C) or the CPMV-EV control (D).

(E) to (G) Sections probed with antisera raised against UGT74H5.

(E) Wild-type oat root tip epidermis.

(F) and (G) N. benthamiana leaves expressing CPMV-AsUGT74H5 (F) or CPMV-EV (G).

(H) and (I) Sections probed with antisera raised against SCPL1. Wild-type (H) or sad7 (#616) (I) mutant oat root tip epidermis.

Gold particle labeling is visible as black dots. Selected subcellular compartments are labeled as follows: C, cytoplasm; V, vacuole; P, plastid; M, mitochondria; and W, cell wall. Images are representative of 10 to 20 biological replicates, and quantification of gold particle densities in different compartments is presented in Supplemental Table 2 online. Bars = 0.5 μm.