Figure 4.

Female Reproductive Organs Are Defective in pat10.

(A) and (B) Aniline blue staining of emasculated wild-type pistils (A) and pat10 pistils (B) hand-pollinated with wild-type pollen at 24 HAP. Arrows indicate the growth fronts of pollen tubes.

(C) and (D) Scanning electron microscopy of a mature ovule from the wild type (C) and pat10 (D).

(E) to (H) Optical sections of wild-type ovules from unfertilized mature pistils (E) and from pistils at 9 HAP (F), 24 HAP (G), and 48 HAP (H). Dotted lines highlight the embryo sacs. Arrows indicate the chalazal end.

(I) to (L) Optical sections of pat10 ovules from unfertilized mature pistils (I) and from pistils at 9 HAP (J), 24 HAP (K), and 48 HAP (L). Dotted lines highlight the embryo sacs. Arrows indicate the chalazal end.

Bars = 200 µm in (A) and (B), 20 µm in (C) and (D), 25 µm in (E) and (I), and 50 µm in (F) to (H) and (J) to (L).