Table 1. Loss of Function of PAT10 Resulted in Defective Male Transmission.
| Experiment Parent | F1 Segregation | ||
|---|---|---|---|
| Female × Male | Genotype | Expected Ratio | Observed |
| 1. pat10-1+/− × wild type | PAT10+/+: +/− | 1:1 | 90:98a |
| 2. Wild type × pat10-1+/− | PAT10+/+: +/− | 1:1 | 121:7b |
| 3. pat10-2+/− × wild type | PAT10+/+: +/− | 1:1 | 96:71a |
| 4. Wild type × pat10-2+/− | PAT10+/+: +/− | 1:1 | 160:55b |
| 5. pat10-1+/− × pat10-1+/− | PAT10+/+:+/−:−/− | 1:2:1 | 115:208:50c |
| 6. pat10-2+/− × pat10-2+/− | R:Sd | 3:1 | 507:228e |
a
Not significantly different from the segregation ratio 1:1 (Student’s t test, P > 0.05).
b
Significantly different from the segregation ratio 1:1 (Student’s t test, P < 0.01).
c
Significantly different from the segregation ratio 1:2:1 (Student’s t test, P < 0.01).
d
R for Basta resistant and S for Basta sensitive. The mutant copy of pat10-2 is resistant to Basta salt; therefore, Basta resistance was calculated here.
e
Significantly different from the segregation ratio 3:1 (Student’s t test, P < 0.01).