Figure 7.

TFL1 Rises with FT Levels.

Quantitative PCR was used to analyze how TFL1 and FT expression varies upon the floral transition in the whole rosette. Plants were grown for 20 d under noninductive 8-h short days and shifted into inductive 16-h long days to trigger flowering. Samples were taken immediately before shifting and every day following the shift for 5 d at dusk. This observation confirms as indicated by in situ hybridization (Figures 2J and 2K) that TFL1 expression rises in proportion to the floral inductive signal (FT).