Role of Phytochromes in Branch Suppression during Shade Avoidance.
(A) Shoot branching phenotype of phytochrome mutants. Number of RI relative to the number of rosette leaves is represented (n = 20 to 22). phyAD, phyA phyD; phyAE, phyA phyE; phyAB, phyA phyB; phyBD, phyB phyD; phyBE, phyB phyE. wt, the wild type.
(B) Shoot branching phenotype of wild-type, phyB, brc1, and phyB brc1 mutant plants (n = 72 to 76).
(C)
BRC1 mRNA levels in axillary buds of wild-type and phyB mutant plants after 8 h of exposure to R or R+FR, analyzed by qPCR. Levels are relative to those of R-treated wild-type plants. Error bars are se of three biological replicates. Different letters denote significant differences (one-way ANOVA, P < 0.05) among means.