Figure 7.

BRC1 Negatively Regulates Bud Activation Genes and Cell Cycle Genes in Seedlings.

(A) and (C) mRNA levels, quantified by qPCR, of bud activation genes in 7-d-old seedlings carrying an estradiol-inducible construct lexa:BRC1 (A) or lexa:GUS (C), 7 h after the beginning of treatment (mock or 10 μM estradiol).

(B) and (D) mRNA levels of selected cell division genes using the same mRNA samples as in (A) and (C), respectively. Error bars are se. Asterisks are significant differences between mock and estradiol-treated plants (Student’s t test, *P < 0.1 and **P < 0.05).

(E) Phenotype of 7-d-old wild-type and lexa:BRC1 seedlings treated with either mock or 10 μM estradiol after germination.

[See online article for color version of this figure.]