Figure 4.

EMP5 Is a Mitochondrion-Localized PPR-DYW Protein.

(A) The EMP5 protein contains 11 PPR motifs and E, E+, and DYW motifs at the C terminus. Locations of four Mu insertion alleles are marked with triangles.

(B) Alignment of 11 PPR motifs found in EMP5 proteins. Identical residues are shaded in yellow and similar residues in light blue.

(C) A EMP5^N469-GFP fusion protein that carried the N terminus 469 amino acids fused with GFP was expressed in transgenic Arabidopsis leaves. The leaf samples and protoplasts were used for GFP signal detection by confocal microscopy. Fluorescent signals from EMP5^N469-GFP are green and MitoTracker stained mitochondria are red. Bar = 10 µm.

(D) Chloroplast import of full-length EMP5 protein into pea chloroplasts. C, chloroplasts after imported with ³H-EMP5; C+T, ³H-EMP5 imported chloroplasts treated with thermolysin to remove surface adhered proteins; TP, translated precursor EMP5 labeled with ³H-Leu.