Figure 5.

High NCED4 Expression Is Associated with Thermoinhibition.

(A) and (B) Seed germination of thermosensitive (Sal, DS, W46, and W55) and thermotolerant (UC, W48, and W51) varieties and accessions at 20 and 35°C in the light (A) or in the dark (B) for 3 d. Closed bars, 20°C; open bars, 35°C.

(C) and (D) Normalized NCED4 mRNA abundance in dry (0 h) and imbibed seeds at 20 or 35°C, respectively. Error bars denote se (n = 3).

(E) Alignment of NCED4 promoters from lettuce genotypes. Boxed sequences are predicted cis-elements based on the PLACE database. NB, no binding site; see the text for descriptions of MYB1AT, ARR1AT, and DOF. The seed germination percentages (Germ) of the genotypes at 35°C are shown at left; W51, W48, and UC are thermotolerant accessions.

[See online article for color version of this figure.]