Table 2. Effect of Silencing of NCED4 on Lettuce Seed Set and Seed Weight.

Line	Seeds per Flower	Weight per Seed	Undeveloped Seeds per Flower
RNAi2-CTL	10.93 ± 0.79	1.11 ± 0.01	6.12 ± 0.29
RNAi8-CTL	12.20 ± 0.79	1.32 ± 0.02	6.05 ± 0.36
RNAi8-7	6.57 ± 0.35**	1.55 ± 0.02**	6.10 ± 0.28
RNAi8-4	6.40 ± 0.38**	1.54 ± 0.03**	6.15 ± 0.29

For seed set, seeds (full and undeveloped) of 15 buds for each replicate were collected for four replicates each from two homozygous RNAi lines (RNAi8-4 and RNAi8-7) and two RNAi control lines (RNAi2-CTL and RNAi8-CTL). For seed weight, 50 mature seeds were weighed for each replicate for each line. Statistical analysis by a one-tailed Student’s t test indicated significant differences (**P < 0.01).