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. 2013 Mar 5;25(3):974–984. doi: 10.1105/tpc.112.108027

Figure 1.

Figure 1.

Functional Interaction between AMT1;3 and AMT1;1 When Coexpressed in Yeast.

(A) The yeast strain DL1 (Δmep1-3 ΔGap1) and DL1 with stable integration of AMT1;3 (Δmep1-3 ΔGap1::AMT1;3) were transformed with the empty vector pDR, pDR-AMT1;3, pDR-AMT1;3TA(T464A), or pDR-AMT1;3TD(T464D).

(B) DL1 strains with stable integration of either AMT1;1 (Δmep1-3 ΔGap1::AMT1;1) or AMT1;3 (Δmep1-3 ΔGap1::AMT1;3) were transformed with the empty vector pDR, pDR-AMT1;3TA(T464A), pDR-AMT1;3TD(T464D), pDR-AMT1;1TA(T460A), or pDR-AMT1;1TD(T460D).

Transformants were selected on YNB medium supplemented with 1 mM Arg. Five microliters of yeast cell suspensions from overnight cultures were spotted in one- to fivefold dilutions on YNB medium supplemented with 2 mM NH4Cl or 1 mM Arg at pH 5.2, and growth was recorded after 4 d at 28°C.