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Split-Ubiquitin Assay for AMT1;1 and AMT1;3 Protein Interactions.
Split-ubiquitin binary assay of AMT1;1 and AMT1;3 on SC medium supplemented with 75 µM Met. Diploid strains containing AMT1;1- and AMT1;3-Cub fusions were tested with a AMT1;1- or AMT1;3-Nub fusion or a wild-type variant of Nub (NubWT).
