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. 2013 Mar 19;25(3):985–998. doi: 10.1105/tpc.113.109256

Figure 4.

Figure 4.

Translationally Repressed MAA7 Transcripts Comigrate with Polyribosomes in Suc Density Gradients.

(A) Typical polyribosome profiles of the indicated strains treated with 150 μg/mL cycloheximide throughout lysis and ultracentrifugation procedures (top panels). M, monoribosomes; Poly, polyribosomes. The distribution of the MAA7, ACT1, and 18S rRNA transcripts in the gradient fractions was examined by slot blot hybridization (bottom panels).

(B) Distribution of ACT1 and MAA7 mRNAs across polyribosome profiles of the CC-124, Maa7-IR44s, and Maa7-IR44s(exp5) strains. The values represent the average of three independent experiments ± se.

(C) Distribution of MAA7 siRNAs and of an endogenous miRNA (miR912) in Suc density gradients of the indicated strains, assessed by RNA gel blot hybridization. Numbers above the lanes indicate pooled gradient fractions.