Figure 5.

The Fraction of MAA7 Transcripts Comigrating with Polyribosomes Is Reduced after Suc Density Gradient Ultracentrifugation of Cell Extracts Subjected to Ribosome Run-Off in the Absence of Cycloheximide.

(A) Typical polyribosome profiles of the indicated strains (top panels). M, monoribosomes; Poly, polyribosomes. The distribution of the MAA7, ACT1, and 18S rRNA transcripts in the gradient fractions was examined by slot blot hybridization (bottom panels).

(B) Distribution of ACT1 and MAA7 mRNAs across polyribosome profiles of the CC-124 and Maa7-IR44s strains. The values represent the average of three independent experiments ± se.