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. 2013 Mar 19;25(3):985–998. doi: 10.1105/tpc.113.109256

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© 2013 American Society of Plant Biologists. All rights reserved.

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Figure 6. — siRNA-Repressed MAA7 Transcripts Are Moderately Depleted from Polyribosomal Fractions after Ribosome Run-Off Assays in the Presence of Low Concentrations of Cycloheximide.

(A) Typical polyribosome profiles of the indicated strains treated with 30 μg/mL cycloheximide throughout lysis and ultracentrifugation procedures (top panels). M, monoribosomes; Poly, polyribosomes. The distribution of the MAA7, ACT1, and 18S rRNA transcripts in the gradient fractions was examined by slot blot hybridization (bottom panels).

(B) Distribution of ACT1 and MAA7 mRNAs across polyribosome profiles of the CC-124 and Maa7-IR44s strains. The values represent the average of three independent experiments ± se.