Figure 7.

TSβ Protein Synthesis from the MAA7 Transcript, Subjected to siRNA-Mediated Translation Repression, Shows Lower Sensitivity to Inhibition by Cycloheximide.

(A) Growth and survival of the indicated strains on TAP medium without (top panels) or with (bottom panels) 7 μM 5-FI supplemented with solvent (Ctrl) or sublethal concentrations of hygromycin B (HygB; 4.0 μg/mL) or cycloheximide (Chx; 2.0 μg/mL). Maa7-IR5, strain expressing a MAA7 IR transgene that induces target mRNA degradation (Rohr et al., 2004).

(B) Immunoblot analyses of TSβ and histone H3 protein levels. Cells from the indicated strains were cultured for 18 h in liquid TAP medium alone (Ctrl) or containing sublethal concentrations of antibiotics (2.0 μg/mL of hygromycin B or 1.0 μg/mL of cycloheximide). Proteins corresponding to equal numbers of cells were loaded per lane.