Figure 5.

Catalyst-mediated oxidative cleavage of the Fl-RRE RNA was dependent on the presence of redox co-reactants. (A) The dependence of the abundance (MS peak area fractions) of oxidation products (cleavage fragments containing nascent terminal 3’-PO₄, 3’-PG, or 5’-PO₄ overhangs at positions adjacent to each illustrated site of H-abstraction) on the co-reactant combinations used (1 mM each co-reactant), for Fe-EDTA-Rev and for the control reactions lacking catalysts, after 1h reaction. (B) The dependence of the total relative abundance of oxidation products (sequence-summed) on the co-reactant combinations used, for all complexes, after 1h reaction. Three different co-reactant combinations were used: 1 mM H₂O₂ + 1 mM ascorbate (H + A), 1 mM ascorbate (A), or no co-reactants (none). Co-reactant-dependence plots are shown for other catalysts in Figures SM18 to SM25 of the Supporting Information.