Figure 6.

Apparent initial rates of formation of cleavage products were determined for cleavage at all nucleotide positions within the RRE RNA, and for fragments with each type of nascent terminal overhang, following time-dependent cleavage of Fl-RRE RNA by (A) Cu-NTA-Rev, or (B) no catalyst, each in the presence of co-reactants (1 mM ascorbate/H₂O₂). Time-dependent experiments were monitored by MALDI-TOF MS (~ 8 mass spectra per catalyst). Rates for all other M-chelate-Rev complexes are shown in the same format in Figures SM1 to SM12 of the Supporting Information. *The apparent initial rate of disappearance of the full-length Fl-RRE RNA is truncated in the figure for Cu-NTA-Rev.