Figure 7.

Breakdown of the apparent initial rates of formation of RNA cleavage fragments containing each type of nascent terminal overhang, resulting from cleavage of Fl-RRE RNA by each M-chelate-Rev complex, in the presence of co-reactants (1 mM ascorbate/H₂O₂). Time-dependent experiments were monitored by MALDI-TOF MS (typically 8 mass spectra per catalyst). Also included were the apparent initial rates of formation of full-length RNA with either missing bases or one or more additions of oxygen. The quantified nascent terminal overhang types were 3’-OH, 2’,3’-cPO₄, 3’-PO₄, 3’-PG, 3’-a-B, 5’-z, 5’OH, and 5’-PO₄. In order to obtain the apparent initial rate of formation for a given overhang type, the apparent rates of formation for that product type at all positions within the RNA sequence were summed. All rates are listed in Table SM1 of the Supporting Information.