Figure 8.

Correlation between the abundance of 3’-PG overhangs and the abundance of base 2-hydroxypropenals, following 4’-hydrogen abstraction from RNA. Following 1 h reaction of RRE RNA (10 µM) with each M-chelate-Rev catalyst (10 µM) and the co-reactants ascorbate and H₂O₂ (1 mM each), the apparent abundance of fragments containing nascent 3’-PG overhangs was determined by MALDI-TOF MS (y-axis), and the relative abundance of 2-hydroxypropenals was determined by the TBA assay (x-axis). Each point above represents a unique catalyst, and the colors shown correspond to the metal in each catalyst: orange, Fe; pink, Co; green, Ni; blue, Cu. The limit of detection for the TBA assay was 3.6 fluorescence intensity units.