Table 1.
Putative proteins that may phosphorylate YB-1 at Ser, Thr, and Tyr suggested by the Motif Scanning Prediction Tool.
| YB-1 Domain | Residue | Pitative Regulatory Protein | Percentile1 |
|---|---|---|---|
| AP-rich N-Term | Ser 3 | Casein Kinase 2 | 3.764 |
| Thr 7 | DNA PK | 0.846 | |
| ATM kinase | 4.202 | ||
| Ser 21 | GSK3 Kinase2 | 3.336 | |
| Thr 29 | PKC δ | 0.806 | |
| Ser 32 | Casein Kinase 1 | 4.315 | |
| Ser 36 | Erk 1 Kinase 2 | 3.248 | |
| cold shock domain | Thr 62 | PKC δ | 2.773 |
| PKC α/β/γ | 4.452 | ||
| PKC θ | 4.964 | ||
| Thr 80 | DNA PK | 2.965 | |
| Thr 89 | Calmodulin dependent kinase2 | 2.786 | |
| PKC θ | 4.964 | ||
| Ser 102 | Akt kinase3 | 2.703 | |
| Ser 102 | PKC ε | 3.527 | |
| Glu 107 | PDZ (nNOS)class 3 | 1.003 | |
| Thr 108 | DNAPK | 3.529 | |
| Casein kinase 2 | 3.764 | ||
| c-term | Tyr 162 | Grb2 SH2 | 2.274 |
| Ser 167 | ATM kinase | 4.812 | |
| Ser 176 | 14-3-3 | 1.029 | |
| Casein kinase 2 | 2.023 | ||
| Tyr 197 | Shc PTB | 3.945 | |
| p85 SH2 | 4.884 | ||
| Tyr 208 | Abl SH2 | 0.292 | |
| Crk SH2 | 1.209 | ||
| Itk SH2 | 2.124 | ||
| Nck SH2 | 2.092 | ||
| Grb2 SH2 | 2.983 | ||
| Thr 271 | DNA PK | 1.761 | |
| ATM kinase | 2.342 | ||
| Tyr 287 | Shc PTB | 3.170 | |
| Ser 314 | Casein kinase 2 | 2.775 |
1
Given by the Motif scanning prediction tool, the percentile tells how the protein ranks by comparing with all vertebrate proteins in Swiss-Prot, the sequence surrounding that site, and the solvent accessibility at that position.
2
Phosphorylation of YB-1 by GSK3 and ERK2 has been demonstrated by Coles et al. 2005.
3
Phosphorylation of YB-1 by Akt has been shown by Sutherland et al. 2005 and Evdokimova et al. 2006.