Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Apr 24;8(4):e62855. doi: 10.1371/journal.pone.0062855

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Yang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Following isolation, splenocytes were stained and sorted first for CD19⁺ B cells. Then CD5⁺CD1d^high B cells were analyzed in a CD19⁺ gate by flow cytometry (left). The percentages of CD5⁺CD1d^high cells among CD19⁺ B cells are shown (right, n = 6 for each group). (B) A positive correlation between the proportions of CD4⁺CXCR5⁺PD-1⁺ T cells and CD19⁺CD5⁺CD1d^high B cells in spleens of MRL/lpr mice (n = 6) was found. (C) Splenocytes were isolated from MRL/lpr and B6 mice and stimulated with LPS plus PIB for 5 hours. CD19⁺IL-10⁺ cells among CD19⁺ B cells were detected by intracellular cytokine staining and flow cytometry analysis (n = 6 for each group). (D) Sorted CD19⁺CD5⁺CD1d^high B cells from MRL/lpr and B6 mice were stimulated with LPS for 48 hours and PIB for the last 5 hours, IL-10 mRNA expression was detected by real-time RT-PCR. Results shown are representative of at least three independent experiments. (E) IL-10 protein expression in spleens was confirmed by immunohistochemical staining. Further magnification of the black-bordered box shows the predominance of IL-10⁺ lymphocytes. The scale bar represents 50 μm. (F) A positive correlation between the numbers of IL-10⁺ and IL-21⁺ cells in spleens of MRL/lpr mice (n = 6) was observed. (G) A positive correlation between the serum levels of IL-21 and IL-10 in MRL/lpr mice (n = 6) was found. (H) The percentage of CD19⁺IL-10⁺ cells among B cells in spleens of MRL/lpr mice with neutralization of IL-21 or PBS vehicle control once per week for 4 weeks (n = 6) was analyzed by flow cytometry. (I) The concentrations of IL-10 in supernatants of cultured B6 mouse-derived B cells that were induced for 3 days by LPS plus 20% of supernatants of Tfh cell cultures from MRL/lpr (mTfh) and B6 mice (bTfh) or vehicle (culture media with 2 µg/ml plate-bound anti-CD3 and 2 µg/ml soluble anti-CD28) with or without neutralization of IL-21 and stimulated with PI for the last 5 hours were determined. Results shown are representative of at least three independent experiments.