Skip to main content
. 2013 Apr 24;8(4):e62016. doi: 10.1371/journal.pone.0062016

Figure 9. Activation of NF-κB proteins by LPS and zymosan.

Figure 9

(A) Macrophages were treated for up to 5 h with 10 ng/ml LPS and RelA/p65 was assayed in the cytoplasm and nucleus. (B) Macrophages were primed for 3 h with LPS and then stimulated for different times. (B–C) The nuclear fractions were used for the assay of RelA/p65, c-Rel, and p50. (D) Binding of RelA/p65 and c-Rel, and histone H3 acetylation of the ptgs2 promoter were assayed in the presence and absence of LPS priming 2 h after stimulation. These are representative experiments of at least two showing identical results.