Table 1. Oligonucleotide primers used for real time RT-PCR and ChIP.
Gene | Sequence (5′-3′) | GeneBank AC |
clec7a (dectin-1) exon 1 forward | GGGCTCTCAAGAACAATGGA | AF400596 |
exon 6 reverse | TTGGAGATGGGTTTTCTTGG | |
exon 5 reverse | CCCAGAGCCATGGTACCT | |
CD209 (DC-SIGN) forward | AGGTCCCCAGCTCCATAAGT | NM_021155 |
reverse | TCTCTGGAAGCTCACCCACT | |
tlr2 (TLR2) forward | GCCAAAGTCTTGATTGATTGG | NM_003264 |
reverse | TTGAAGTTCTCCAGCTCCTG | |
mrc1 forward | GCTGAACCTGGAAAAAGCTG | NM_002438 |
reverse | ACGAAGCCATTTGGTAAACG | |
gapdh forward | GTCAGTGGTGGACCTGACCT | NM_002046.3 |
reverse | AGGGGAGATTCAGTGTGGTG | |
ptgs2 promoter forward | AGGAGAGGGAGGGATCAGAC | D28235 |
reverse | TTTACCCACGGAAATGAGAAA |
mrc1 stands for the mannose receptor gene.