Table 5. qCR validation.
Gene Name | Fold Change | ||||
Inflammatory chemokines | LPS | CBD+LPS | THC+LPS | CBD | THC |
Ccl2 | 5.2±1.5 | 2.8±0.3 | 5.7±1.3 | 0.5±0.08 | 1.2±0.1 |
Ccl12 | 10.7±2.5 | 3.8±0.4 | 5.8±1.2 | 0.8±0.2 | 0.9±0.1 |
Cxcl10 | 52.7±3.2 | 41.2±5.1 | 45.1±2.8 | 1.8±0.3 | 0.7±0.1 |
Membrane transport and secretion | |||||
Aqp9 | 2.5±0.3 | 34.7±7.1 | 6.8±1.6 | 14.6±3.8 | 2.5±0.5 |
Mcoln2 (TRPML2) | 3.9±0.3 | 5.3±0.1 | 4.0±0.6 | 1.5±0.8 | 1.6±0.5 |
Slc7a11 | 6.5±0.3 | 19.8±0.1 | 15.3±0.1 | 9.2±0.6 | 1.4±0.2 |
Cell cycle and proliferation | |||||
Cdkn1a/p21 | 7.3±2.1 | 12.6±2.2 | 6.2±0.7 | 3.2±0.6 | 1.0±0.1 |
Phosphatases | |||||
Dusp1 | 2.4±0.2 | 9.1±1.8 | 2.2±0.4 | 3.2±1.5 | 0.8±0.1 |
Dusp2 | 2.5±0.2 | 1.1±0.5 | 2.2±0.2 | 0.8±0.2 | 0.9±0.2 |
Regulatory molecules | |||||
Sqstm1/p62 | 3.0±0.8 | 5.9±1.8 | 3.2±0.8 | 4.8±1.7 | 1.7±0.1 |
G protein-coupled receptors | |||||
Ebi2/Gpr183 | 0.3±0.1 | 0.06±0.02 | 0.08±0.01 | 0.24±0.01 | 1.06±0.19 |
Ptgir | 5.3±0.7 | 13.4±0.8 | 7.7±0.6 | 11.9±0.6 | 1.6±0.2 |
GPR55 | 0.19±0.04 | 0.15±0.03 | 0.29±0.07 | 0.83±0.06 | 1.0±0.2 |
Cnr2 | 0.1±0.01 | 0.1±0.02 | 0.15±0.02 | 0.9±0.1 | 1.05±0.15 |
Host defense and adaptive response | |||||
Tlr2 | 4.1±0.9 | 2.6±0.3 | 3.2±0.6 | 1.1±0.1 | 1.1±0.2 |
Stress response | |||||
Trib 3 | 0.6±0.1 | 15.2±0.4 | 1.6±0.2 | 15.7±0.3 | 3.6±0.9 |
Hmox1 | 0.84±0.04 | 3.6±0.9 | 0.9±0.2 | 3.9±1.2 | 2.1±0.2 |
Transcription factors | |||||
Pparg1 | 0.14±0.01 | 0.27±0.06 | 0.12±0.03 | 1.4±0.3 | 1.27±0.01 |
Pparg2 | 0.15±0.02 | 0.06±0.01 | 0.35±0.06 | 1.6±0.2 | 1.0±0.1 |