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. Author manuscript; available in PMC: 2014 Mar 21.
Published in final edited form as: J Phys Chem B. 2013 Mar 8;117(11):3145–3153. doi: 10.1021/jp401806g

Figure 2.

Figure 2

Effect of the buffer ionic strength on modulation depths in the Gd(III)–nitroxide DEER experiment. (a) Primary DEER data and (b) background-corrected form factor traces as calculated by DeerAnalysis 2011 for 109 [Gd(DTPA)]− –68 K1. Results obtained using a 25 mM NaCl-containing buffer and a 500 mM NaCl plus 10% v/v glycerol-containing buffer are displayed in red and blue respectively. A significant increase of the Gd(III)-nitroxide DEER modulation depth is observed. Q-band DEER measurements were recorded at 10 K with a pump pulse flip angle θ = π.