Figure 2. Immunization Schedule and Induction of specific anti-ASP2 humoral immune response in mice vaccinated with recombinant viruses.

Timeline representation of immunization schedule and experimental procedures (A). C57BL/6 mice were immunized as described in Material and Methods. Two weeks after the boost immunization, the animals were bled and the presence of specific anti-ASP2 total IgG antibodies in mice sera was evaluated by western by incubating individual (lanes 1–9) or pooled (lanes 10 and 11) sera of C57BL/6 mice with nitrocellulose membranes loaded with recombinant ASP2 protein (His65KDa) as capture antigen blot (B). Alternatively, the antibodies levels were measured by ELISA using individual sera of C5BL/6 (C) or C3H/He (D) mice sera diluted 1∶100 and recombinant ASP2 protein as capture antigen. Optical Density (OD) was measured at 450 nm.