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. 2013 Apr 24;8(4):e61795. doi: 10.1371/journal.pone.0061795

Figure 3. Cellular responses to immunodominant epitopes from ASP2 in mice immunized with recombinant viruses.

Figure 3

C57BL/6 and C3H/He mice were immunized as described in Material and Methods. Three weeks after the boost immunization, the presence of ASP-2 specific IFN-γ producing T cells in spleen cells of C57BL/6 (A) or C3H/He (B) mice were assessed by ELISPOT and culture supernatant ELISA (n = 8). To this aim, the spleen cells of individual mice were stimulated 18 hours (ELISPOT) or 72 hours (ELISA) ex vivo with VNHRFTLV (aa 553–560; for C57BL/6) or TEWETGQI (aa 320–327; for C3H/He) specific ASP2 peptides. Optical Density (OD) was measured at 450 nm.