Figure 5. Phenotype of anti-ASP2 specific CD8+ T cells elicited by vaccination with recombinant viruses.

C3H/He mice were immunized with recombinant viruses as described in Material and Methods. Two weeks after the last immunization, the spleen cells were harvested and cultivated ex vivo with specific TEWETGQI CD8+ T peptide and incubated with anti-CD3, anti-CD8, permeabilized and fixed and stained with anti-CD107a, anti-IFN-γ and anti-TNF-α antibodies and assessed by flow cytometry. Percentage of effector CD8+ T cells reacting to the presence of TEWETGQI peptide obtained from spleen cells of mice immunized with recombinant viruses (A). Percentage of CD8 T cells which produces IFN-γ or/and TNF-α or/and mobilizes the degranulation marker CD107a after stimulation with TEWETGQI (B), the statistics depicted are compared to groups of mice immunized with control recombinant viruses. The number and frequency of dextramer positive CD8+ T found in 3×10⁴ CD8+ T (C). Functional profile of CD8+ T cells subpopulations obtained from mice immunized with recombinant viruses (D). Response were depicted with different color patterns according to the number of assessed functions (IFN-γ, TNF-α and CD107a) displayed by each dextramer negative or dextramer positive CD8+ T cells subpopulations.