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. 2013 Apr 24;8(4):e61773. doi: 10.1371/journal.pone.0061773

Figure 3. Trypsin treatment does not compromise epidermal barrier integrity.

Figure 3

Ddc.47 is a fluorescent reporter that includes a wound-induced DNA enhancer from the Ddc locus. Confocal images of Ddc. 47 (green) embryos injected with fluorescent Rhodamine Dextran (red) to assess epidermal integrity and reporter activation after trypsin puncture wounding. (A, B) Perivitelline injection of trypsin along with Rhodamine Dextran globally activates the Ddc.47 wound reporter without compromising the epidermal barrier since Rhodamine Dextran is limited to the perivitelline space. (C, D) Embryos punctured with trypsin and Rhodamine Dextran globally activate Ddc.47 wound reporter, but epidermal integrity is lost as Rhodamine Dextran is observed within the embryonic body cavity. (E, F) Control embryos that have been injected in the perivitelline space with Rhodamine Dextran in carrier solution do not activate the Ddc.47 wound reporter. Arrows mark the wound site. Dashed lines in the data panels mark the outlines of embryos.