Figure 8. Abundant overlap of differentially regulated genes after puncture and trypsin puncture wounding.
At each of the three time points (30 minutes, 60 minutes, and 120 minutes) after puncture and trypsin puncture wounding, a comparison of the statistically significant (FDR <0.01) regulated genes was performed using Microsoft Excel software. (A) 30 minutes after puncture and trypsin puncture wounding, 15 and 112 significant genes respectively, were upregulated 1.8 fold or greater; 14 genes were commonly upregulated after both wounding treatments. 60 minutes post puncture and trypsin puncture wounding, 64 and 332 significant genes respectively, were upregulated 1.8 fold or greater; 58 genes were commonly upregulated after both wounding treatments. 120 minutes post puncture and trypsin puncture wounding, 210 and 624 significant genes respectively, were upregulated 1.8 fold or greater; 186 genes were commonly upregulated after both wounding treatments. (B) 30 minutes post puncture and trypsin puncture wounding, 389 and 378 significant genes respectively, were downregulated −1.8 fold or lower; 333 genes were commonly down regulated after both wounding treatments. 60 minutes post puncture and trypsin puncture wounding, 496 and 508 significant genes respectively, were downregulated −1.8 fold or lower; 429 genes were commonly downregulated after both wounding treatments. 120 minutes post puncture and trypsin puncture wounding, 698 and 826 significant genes respectively, were downregulated −1.8 fold or lower; 624 genes were commonly downregulated after both wounding treatments.