Figure 9. Novel localized epidermal, global epidermal, and fat body wound response genes in late stage Drosophila embryos.

Alkaline phosphatase in situ hybridization with probes targeting RNA of candidate wound response genes. Puncture wounded and wild-type stage 15–17 embryos were compared for tissue-specific transcript induction one hour after wounding. (A) Ady43A transcripts accumulate in broad zone in the epidermis around puncture wound sites. (B) Ets21C transcripts are observably upregulated in the narrow zone wound site, and are already present in unwounded embryos at an easily detectable level throughout the entire epidermis. (C) An increase in jra/jun transcripts is observed in a narrow zone around the epidermal wound site. (D) An increase in kay/fos transcripts are also detected in a moderately broad zone around the epidermal wound site. (E) Spz transcripts are detected in a broad zone around the epidermal wound site. (F) After puncture wounding, dorsal transcripts are detected in a moderately broad zone around the epidermal wound site. (G) After puncture wounding, rhomboid transcripts are detected around the epidermal wound site. (H) After puncture wounding Rel RNA is upregulated around the epidermal wound site and in the fat body. (I) IM1 RNA is upregulated throughout the fat body after puncture wounding, but not in the epidermis. (J) NijA RNA is upregulated throughout the entire epidermis after puncture wounding. Embryo bodies are outlined with dashed lines. The puncture wound site is indicated with a red asterisk. Black arrows point to fat body expression.