Table 2. Select upregulated genes from the trypsin puncture wound microarrays.
| CG # | Gene symbol | Protein type/Process | 30 min Fold change | 60 min Fold change | 120 min Fold change | CG # | Gene symbol | Protein type/Process | 30 min Fold change | 60 min Fold change | 120 min Fold change |
| Cuticle Regeneration/Chitin metabolism (10/19) | Epidermal Wound Response (7/10) | ||||||||||
| CG13224 | Cpr47Eb | cuticle protein | 4.2 | 18.3* | 29.8* | CG11086 | Gadd45 | damage inducible protein | 15.3* | 7.6* | 6.5* |
| CG9077 | Cpr47Ec | cuticle protein | 2.0 | 11.9* | 23.3* | CG2914 | Ets21C | ETS transcription factor | 1.9 | 5.4* | 4.9* |
| CG7539 | Edg91 | cuticle protein | 2.8 | 7.4 | 12.3* | CG33956 | kay | FOS transcription factor | 2.6 | 4.5* | 2.9 |
| CG10118 | ple | cuticle regeneration | 5.6* | 12.9* | 9.5* | CG2275 | jra | JUN transcription factor | 1.5 | 2.4 | 2.3* |
| CG1864 | Hr38 | cuticle development | N/A | 6.6* | 8.1* | CG7873 | Src42A | protein tyrosine kinase | N/A | 1.7 | 2.1 |
| CG9076 | Cpr47Ed | cuticle protein | N/A | 3.0 | 8.1 | CG7850 | puc | JNK pathway phosphatase | 1.4 | 2.1 | 1.7 |
| CG10697 | Ddc | cuticle regeneration | 2.0 | 4.3* | 5.3* | CG10244 | Cad96Ca | Stitcher RET RTK | N/A | 1.8 | N/A |
| CG2666 | kkv | chitin synthase | N/A | 2.3 | 3.1 | Cytoskeleton/Cell adhesion (6/12) | |||||
| CG33983 | obst-H | chitin binding protein | N/A | N/A | 2.3 | CG6449 | NijA | cell adhesion | N/A | 2.5 | 4.1* |
| CG15378 | lectin-22C | chitin metabolism | N/A | N/A | 1.8 | CG8095 | scb | cell adhesion molecule | 1.5 | 3.4 | 3.8 |
| Melanization (5/5) | CG5730 | AnnIX | actin binding | 2.1* | 2.3 | 2.7* | |||||
| CG9733 | CG9733 | protease, proAE | 6.0 | 14.1* | 18.9* | CG12051 | Act42A | actin | N/A | N/A | 2.1 |
| CG1689 | lz | crystal cell differentiation | N/A | 8.7* | 14.0* | CG4755 | RhoGAP92B | Rho GTPase activator | 1.4 | N/A | 1.7 |
| CG1102 | MP1 | protease/activator | 1.7 | 2.3 | 3.7 | CG4027 | Act5C | actin | N/A | N/A | 1.7 |
| CG3066 | Sp7 | protease/activator | N/A | 1.7 | 3.4* | Detoxification/Defense/Stress response (8/30) | |||||
| CG11331 | Spn27A | serpin | N/A | 1.7 | 2.1 | CG4421 | GstD8 | glutathione S transferase | 1.7 | 7.5 | 12.8 |
| Innate immunity (29/31) | CG3666 | Tsf3 | iron sequestration | 4.8* | 12.2* | 9.8* | |||||
| CG18372 | AttB | AMP (bacterial) | 43.7* | 120.1* | 73.6* | CG4371 | GstD7 | glutathione S transferase | N/A | 3.3 | 7.5* |
| CG15066 | IM23 | putative AMP | 21.5* | 83.6* | 56.2* | CG10363 | TepIV | humoral response | 1.7 | 3.4* | 4.2* |
| CG18106 | IM2 | putative AMP | 32.5* | 76.1* | 49.1* | CG7052 | TepII | opsonization | N/A | 2.1 | 3.2 |
| CG4740 | AttC | AMP (bacterial) | 3.4 | 10.7* | 44.1* | CG8913 | Irc | Immune regulated catalase | 1.6 | 1.9 | 3.0* |
| CG10146 | AttA | AMP (bacterial) | 11.6 | 50.2* | 40.3* | CG6965 | mthl5 | GPCR | 1.6 | 3.5* | 2.5 |
| CG10810 | Drs | AMP (fungi) | 5.8* | 27.2* | 39.0* | CG4026 | IP3K1 | oxidative stress response | 2.6 | 1.7* | 2.1* |
| CG18108 | IM1 | putative AMP | 21.4* | 57.2* | 38.9* | Signaling/Miscellaneous (12/25) | |||||
| CG16844 | IM3 | putative AMP | 19.9* | 48.7* | 27.4* | CG1851 | Ady43A | adenosine kinase activity | 15.3* | 40.2* | 9.2* |
| CG15231 | IM4 | putative AMP | 20.7* | 41.9* | 28.0* | CG33542 | upd3 | JAK-STAT signaling | N/A | 5.6* | 4.6* |
| CG8175 | Mtk | AMP (fungi) | 5.1 | 12.4* | 25.9* | CG5993 | os | JAK-STAT signaling | 2.7 | 5.8* | 4.2* |
| CG18279 | IM10 | putative AMP | 11.2* | 27.6* | 18.4* | CG9811 | Rgk1 | GTPase-mediated signaling | 1.9* | 1.8* | 3.6* |
| CG10794 | DptB | AMP (GP bacteria) | 5.0 | 6.6* | 7.5* | CG33338 | p38c | MAP kinase | 1.6 | 2.3 | 3.4 |
| CG11992 | Rel | NFkB transcription factor | 3.4 | 7.9* | 6.4* | CG7450 | CrebA | DNA binding | 2.5* | 2.7* | 2.6* |
| CG11709 | PGRP-SA | PRR | 1.5 | 2.7 | 6.2* | CG5248 | loco | GPCR signaling | 1.4* | 2.1* | 2.3* |
| CG1857 | nec | serpin/Toll signaling | 2.4 | 3.8* | 6.1* | CG6117 | Pka-C3 | protein kinase | N/A | N/A | 2.3 |
| CG4437 | PGRP-LF | PRR | 7.3* | 10.7* | 5.5* | CG1147 | NPFR1 | Neuropeptide Y receptor | N/A | N/A | 2.2 |
| CG6134 | spz | Toll signaling ligand | 1.8 | 4.6 | 5.5* | CG6103 | CrebB-17A | DNA binding | 1.4 | 1.6 | 2.2 |
| CG16705 | SPE | protease/Toll signaling | 1.7 | 2.9* | 4.0* | CG4472 | Idgf1 | imaginal disc development | 1.4 | 2.3 | 2.2* |
| CG2056 | spirit | protease/Toll signaling | 1.5 | 2.2 | 3.5* | CG1004 | rho | EGFR pathway activator | N/A | 1.8 | N/A |
| CG14704 | PGRP-LB | PRR | 2.0 | 4.7* | 3.4* | Serine proteases & Serpins (7/16) | |||||
| CG32042 | PGRP-LA | PRR | 1.3 | 1.9 | 2.8* | CG33329 | Sp212 | serine protease | 1.5 | 3.6 | 7.6* |
| CG5848 | cact | protein binding/Toll signaling | 1.4 | 2.0 | 2.5 | CG2045 | Ser7 | serine protease | N/A | 4.1* | 6.8* |
| CG1165 | LysS | lysozyme activity | N/A | N/A | 2.6 | CG18525 | Spn5 | serpin | 1.5 | 2.7 | 4.9* |
| CG1365 | CecA1 | AMP (bacterial) | N/A | 1.7* | 2.5* | CG4821 | Tequila | Neurotrypsin ortholog | N/A | N/A | 3.9* |
| CG4432 | PGRP-LC | PRR | 2.5* | 2.6* | 2.4* | CG10913 | Spn6 | serpin | N/A | 2.1 | 3.3 |
| CG6367 | psh | protease/Toll signaling | 1.5 | 1.8* | 2.4* | CG7996 | snk | protease/Toll signaling | N/A | 1.6 | 2.4* |
| CG6667 | dl | NFkB transcripton factor | N/A | 2.7 | 2.1* | CG9453 | Spn4 | serpin | 1.4 | 2.1 | 2.9* |
| CG1373 | CecC | AMP (bacterial) | N/A | 1.8* | 2.1* | ||||||
| CG6794 | Dif | NFkB transcripton factor | N/A | 1.6 | N/A | ||||||
A total of 84 significantly upregulated genes after trypsin puncture wounding were manually classified into the above labeled categories. The categorized genes are based on 120 minute significant fold change values since this timepoint contained the highest amount of upregulated genes after either wounding treatment. Category headings denote the number of genes manually listed in the table out of the total number of upregulated genes 120 minutes after trypsin treatment that fall into the same category heading. “CG #” refers to the accession numbers from Flybase. “Gene symbol” refers to gene symbol on Flybase. “Protein type/Process” refers to experimentally verified or putative functions assigned to genes. “Fold change” refers to fold changes seen in gene expression values (either puncture or trypsin puncture treatments) relative to wild-type untreated values. All genes shown are statistically significant and have a FDR value of less than 0.01. PRR, Peptidoglycan Recognition Receptor. N/A indicates that a statistically significant fold change was not achieved for the corresponding gene at that specific timepoint or that duplicate probe values were not reproducible. *Denotes gene that is significantly upregulated at corresponding timepoint after puncture wounding with a FDR less than 0.01.