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. 2013 Apr 24;8(4):e61836. doi: 10.1371/journal.pone.0061836

Figure 5. E2F1 overexpression increases the survival of NSAID treated ovarian cancer cells.

Figure 5

A) HEY cells were transiently transfected with E2F1 or eGFP as a control and treated for 24 hours with 300 µM diclofenac or indomethacin. Cell lysates were analyzed by immunoblotting with the indicated antibodies. B) HEY cells were transiently transfected with E2F1 or eGFP as a control and treated for 48 hours with 300 µM diclofenac or indomethacin. Cells were then washed and allowed to grow and stained with crystal violet. Image shown is representative of 2 experiments. C) HEY cells were treated for 24 hours with 300 µM diclofenac or indomethacin. Cell lysates were analyzed by immunoblotting. D) HEY cells were transfected with Rb siRNA and treated for 24 hours with diclofenac or indomethacin. Cell lysates were analyzed by immunoblotting. E) HEY cells were transfected with Rb siRNA and treated for 48 hours with 300 µM diclofenac or indomethacin, cells were then washed and allowed to grow and stained with crystal violet. Image shown is representative of 3 experiments.