Figure 2. H(H₂O)_m prevents and UV-induced MMP-1, COX-2, IL-6 and IL-1β mRNA expression in young human skin in vivo.

Skin from young human buttocks was irradiated with UV light and then locally treated with or without H(H₂O)_m for 2 hr. Twenty-four hours after irradiation, and then skin was biopsied. (A) MMP-1, (B) COX-2, (C) IL-6 and (D) IL-1β mRNA expressions were determined by real time RT-PCR. Results are expressed as means ± SEM with scatter plots (n = 11), * p<0.05, ** p<0.01, *** p<0.001. (E) Immunohistochemical staining was performed using anti-human MMP-1 antibody. The figures shown are representative of eleven subjects.