Figure 7. Basal expression of MMP-1, COX-2 and IL-6 are decreased by H(H₂O)_m treatment in intrinsically aged human buttock skin and H(H₂O)_m increases basal expression of type I procollagen expression in photoaged facial skin and also reduces the basal expressions of MMP-1 and IL-6.

(A) Aged human buttock skin was topically treated with or without H(H₂O)_m using a releasing device for 2 hr. MMP-1, COX-2 and IL-6 mRNA expressions were determined by real time RT-PCR. Results are shown as means ± SEM with scatter plots (n = 7), * p<0.05. (B) Immunohistochemical staining was performed using anti-human MMP-1 antibody. The figures shown are representative of seven subjects. (C) Aged human face skin was topically treated with or without H(H₂O)_m for 30 min a day for 4 days. Type I procollagen, MMP-1 and IL-6 mRNA expressions were determined by real time RT-PCR. Results are expressed as means ± SEM with scatter plots (n = 10), * p<0.05, *** p<0.001.