Figure 8. Drosophila homologues of ITGAM, If and mew do not behave as biomarkers in flies.

(A–C) A cartoon of idealized data of expected transcriptional patterns exhibited by biomarkers of sleepiness for comparison to empirical data in D–I. D, G) Transcripts for if and mew are not elevated in Cs flies when waking is induced either by caffeine (C) (which is accompanied by a sleep rebound) or methamphetamine (M) (which is not followed by an increase in sleep) compared to untreated siblings. (E,H) cyc⁰¹ mutants do not show an increase in if or mew transcripts following sleep deprivation. (F,I) There is no step increase in if or mew transcripts following 6 or 9 h of sleep deprivation in tim⁰¹ mutants.