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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Clin Immunol. 2013 Mar 6;147(2):105–119. doi: 10.1016/j.clim.2013.02.015

Figure 6. Significant improvement in CD8+ T cell suppressive potential within 12h of GA therapy initiation.

Figure 6

Ex vivo purified CD8+ cells were used in anti-CD3-stimulated CFSE-based suppression assays using third-party Teff cells and irradiated APC. Panels A and B show representative dot plots from a GA-treated patient at 0h and 12h, respectively. Percentages in dot plot indicate gated percentages of CD4+/CFSE low (proliferating) cells. Percent proliferative responses were normalized to the response without suppressors (defined as 100%), indicated as 1:0 in the dot plots. Panel B represents %suppression by CD8+ T cells isolated from HD, untreated, and GA-treated MS patients. GA-treated patients showed significant improvement in CD8+ T cell mediated suppression at 12h (D). No such change was seen in HD and untreated MS patients. Per patient trends in GA treated group at 12h are shown in D.