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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Clin Immunol. 2013 Mar 6;147(2):105–119. doi: 10.1016/j.clim.2013.02.015

Figure 7. Inhibitory potential of induced suppressors is significantly improved at 12h after GA treatment.

Figure 7

Induced suppressors were generated by activating CD4+CD25− T cells in the presence of anti-CD3 and allogeneic APC for 6 days. These cells were harvested and assessed for suppressive ability in CFSE-based suppression assays using third-party Teff cells and irradiated-APC. Representative dot plots (A and B) shows %proliferating Teff cells when cultured with induced suppressors obtained from a GA-treated patient at 0 and 12h time points. Percent proliferative responses were normalized to the response without suppressors (defined as 100%), indicated as 1:0 in the dot plots. At baseline, induced suppressors generated from MS patients displayed significantly reduced inhibitory potential when compared to HD (C). Data points in D represent % suppression by induced suppressors generated from HD, untreated and GA treated (n=6) MS patients. Induced suppressors generated from CD4+CD25− T cells obtained at 12h after GA therapy showed significant improvement in inhibitory potential (D). No such changes were seen in HD and untreated MS patients. Per-patient trends in GA-treated group are shown in Panel E.