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. Author manuscript; available in PMC: 2014 Apr 23.
Published in final edited form as: ACS Nano. 2013 Mar 5;7(4):3341–3350. doi: 10.1021/nn400125c

Figure 6. Effect of N-ethylmaleimide on formation of the NCp7-SL3 RNA complex.

Figure 6

Event frequency modulation caused by the addition of 5 μM high-affinity SL3 (GAG) aptamer, with 10 μM NCp7, causing reduction in the event frequency. Introduction of 60 μM N-ethylmaleimide returns the event frequency to near its pre-NCp7 level. The applied transmembrane potential was +400 mV. The buffer solution contained 200 mM NaCl, 5mM NaH2PO4, pH 7.0 on the cis side, and 1 M NaCl, 5 mM NaH2PO4, pH 7.0 on the trans side. The nanopore was ~6 nm in diameter in a 15-nm thick nitride.